Ozone Inactivates HIV at Noncytotoxic Concentrations
DT 9104
AU Michael T.F. Carpendale and Joel K. Freeberg
SO Antiviral Research, 16 (1991) pp. 281-292
AB The inactivation of human immunodeficiency virus (HIV) and
cytotoxic properties of ozone-treated serum and serum-
supplemented media were examined. The titer of HIV
suspensions in human serum was reduced in a dose-dependent
manner when treated with total reacted ozone
concentrations at a range of 0.5 to 3.5 ug/ml(-1).
Complete inactivation of HIV suspensions was achieved by
4.0 ug/ml(-1) of ozone in the presence or absence of H-9
cells. In contrast, cellular metabolism, as measured by
MTT dye cleavage, and DNA replication, as measured by BUdR
incorporation, were enhanced in H-9 cells grown in media
treated with quantities of ozone that completely
inactivate HIV. The permissively HIV-infected cell line
HXB/H-9 was cultured in ozone-treated media for six days
with culture supernatants being sampled and assayed on
alternate days for HIV p24 core protein. HIV p24 was
reduced in all treated cultures compared to control
cultures, with an average reduction of 46% [p24].