Ozone may provide Environmentally Safe Protection for Grains
January 30, 2003
WEST LAFAYETTE, Ind. - Taking a clue from air purification systems
used in surgical suites, Purdue University researchers have
discovered that ozone can eliminate insects in grain storage
facilities without harming food quality or the environment.
Ironically, the gas is being touted as a fumigant alternative in
response to an international treaty banning the use of ozone-layer
harming chemicals currently used to rid food storage facilities of
insects. When ozone is used for killing grain insects, it lasts for
a very short period of time without damaging the environment or the
grain, the Purdue scientists report in the January issue Journal of
Stored Products Research.
"Ozone has a very short half-life and we're using relatively low
dosages, but enough to kill an insect," said Linda Mason, Purdue
entomology associate professor and co-author of the study. "The
chemicals currently used can kill everything in and around the grain
bin, including people. With ozone, we're not generating ozone at
deadly concentrations, and we have better control over it when it's
Purdue's Post Harvest Grain Quality Research team began its studies
in response to the 1987 Montreal Protocol, an international
agreement to prohibit substances deemed dangerous to the Earth's
ozone layer. One such substance is methyl bromide, commonly used
against crop pests in the soil and in grain storage facilities.
Beginning in 2005, it no longer will be available.
A replacement for chemical fumigants is imperative because insects
not only eat the grain, they defecate on it causing development of
fungi, primarily Fusarium and Aspergillus. These fungi can release
potentially deadly mycotoxins that can cause illness in most
livestock and have been linked to some forms of human cancer. In
humans, approximately 76 million cases of food-borne disease occur
annually in the United States, according to the Centers for Disease
Control and Prevention.
Experts estimate that 5 percent to 10 percent of the world's food
production is lost each year because of insects, and in some
countries that figure is believed to be as high as 50 percent.
In the latest study, Purdue researchers used ozone to treat rice,
popcorn, soft red winter wheat, hard red winter wheat, soybeans and
corn. They used five-gallon plastic pails and 50-gallon steel drums,
storage bins filled with grain, and buried mesh bags all filled with
grain and a known number of grain-eating bugs to test ozone's
killing efficacy. The team's previous studies on ozone flow and
effectiveness in eliminating insects were done either in similar
storage containers or in 500-bushel bins built for pilot studies at
the Purdue Agronomy Farm.
The ozone treatment of grain included two applications of ozone. In
the first, the ozone moves through the grain slowly because the gas
reacts, or bonds, with matter on the grain surface. This first
treatment allows ozone to react with most of the grain surface and
degrades the ozone back to oxygen, Mason said. With the second ozone application,
the gas moves through the grain more quickly because it isn't slowed
by reactions with the grain. This allows the ozone to kill the
insects by reacting with them rather than the grain.
Testing different grains allowed the scientists to answer two
important questions. One was whether ozone flowed differently
through grains that were less porous or of a different kernel size
than corn, such as wheat. The second was how exposure to ozone
affects the quality of food products made from the treated grain.
Dirk Maier, a Purdue agricultural and biological engineering
professor, studied how to make the ozone flow efficiently and
effectively through grain storage bins. Charles Woloshuk, a botany
and plant pathology professor, studied ozone effects on molds and
mycotoxins. Fidel Mendez, a botany master's degree student, studied
the final products produced from the treated grain to determine if
they were the same quality as those made from untreated grain.
"We wanted to determine if the grain looked any different; if it
milled the same way; if it made flour the same way. Does bread taste
the same when made from ozonated wheat?" Mason said. "Essentially,
there were no differences. The food industry can take grain that's
been treated with ozone and know it won't affect their ability to
come up with the same products in the end."
The team also checked how ozone treatments affected amounts of
important amino acids and essential fatty acids, fats not produced
by the body. The treatments caused no significant difference in any
of the nutritional and metabolic values of these substances in any
of the grains studied, Mason said.
The scientists began their study after a company that uses ozone air
purification systems in hospitals noticed that air vents were
cockroach free. Absence of cockroaches in a large building is
unusual, so the researchers tested various ozone doses on different
insects and found the gas was fatal to bugs.
"All the species we tested seemed affected," Mason said. "The only
ones we don't have control over are immature weevils since they are
hidden within the kernels. Ozone, unlike chemical fumigants, doesn't
penetrate into the kernel enough to kill immature insects."
Currently, the researchers are studying ways to use ozone as a
preventative treatment by possibly sealing of grain storage
facilities with layers of ozone, much the way a jelly jar is capped
The USDA National Research Initiative provided funding for this
Writer: Susan A. Steeves, (765) 496-7481, firstname.lastname@example.org
Source: Linda Mason, (765) 494-4586, email@example.com
Dirk Maier, (765) 494-1175, firstname.lastname@example.org
Ag Communications: (765) 494-2722; Beth Forbes,